• Skip to Content
  • Skip to Main Navigation
  • Skip to Search

Indiana University Bloomington Indiana University Bloomington IU Bloomington

Open Search Menu

The College of Arts & Sciences

Department of Biology

  • Home
  • About
    • Message from the Chair
    • Faculty
    • Research Associates & Postdocs
    • Staff
    • Graduate Students
    • Administration
    • Offices & Centers
    • Diversity & Inclusion
    • Outreach
    • History
    • About Bloomington
  • Undergraduate
    • Biology
    • Biotechnology
    • Microbiology
    • Molecular Life Sciences
    • Research & Teaching Opportunities
    • Honors Program
    • Advising
    • Awards & Scholarships
    • Student Experience
    • Graduate & Professional Program Preparation
    • Career Preparation
  • Graduate
    • Evolution, Ecology, and Behavior Ph.D.
    • Genome, Cell, and Developmental Biology Ph.D.
    • Microbiology Ph.D.
    • Biotechnology M.S.
    • Multidisciplinary & Affiliated Programs
    • Advising
    • Financial Support
    • Awards & Fellowships
    • Life Assistance
    • Career Preparation
    • Alumni Career Spotlights
    • How to Apply
  • Research
    • Research Faculty
    • Associated Research Faculty
    • Research Strengths
    • Centers, Instrumentation, & Resources
    • Faculty Awards
  • News & Events
    • News
    • Events Calendar
    • Seminars
    • Named Lectures
    • Discussion Groups & Journal Clubs
    • Annual GCDB Retreat
    • Annual Microbiology Retreat
    • Newsletters
  • Alumni & Giving
  • Search
  • Contact
  • Student Portal
  • Donate
  • News
  • Events Calendar
  • Seminars
  • Named Lectures
  • Discussion Groups & Journal Clubs
  • Annual GCDB Retreat
  • Annual Microbiology Retreat
  • Newsletters
  • Home
  • News & Events
  • News
  • 2018 News
  • Dalia receives MIRA to study biofilms

Biofilms study seeks novel strategies to combat infections and stem spread of antibiotic resistance

Tuesday, November 13, 2018

Ankur Dalia, 2018.
Ankur Dalia. Photo by James Brosher, IU Communications

The formation of complex multicellular bacterial communities known as biofilms is critical for virulence, environmental persistence, or genetic exchange in microbial pathogens. Thus, understanding the mechanisms underlying biofilm formation may uncover novel approaches to combat diverse clinically relevant infections.

Ankur Dalia, an assistant professor in the Department of Biology, and his lab study the basic biology and mechanisms of biofilms using Vibrio cholerae as a model system. This bacterium is the causative agent of the diarrheal disease cholera; however, rather than studying the virulence of the pathogen, Dalia and researchers in his lab will leverage this well-established model system and the genetic tools they have developed to characterize biofilms in a physiologically relevant context.

V. cholerae forms biofilms in its aquatic reservoir on the chitinous shells of crustacean zooplankton. Chitin biofilms play three important roles in the ecology of this organism. First, V. cholerae degrades chitin into soluble oligosaccharides, which serve as an important carbon and nitrogen source in the aquatic environment. Second, growth in chitin biofilms induces natural transformation, a conserved mechanism of horizontal gene transfer that can promote the acquisition of antibiotic resistance genes and novel virulence factors. Third, chitin biofilms are important for the waterborne transmission of cholera. Following ingestion of a chitin biofilm, V. cholerae must rapidly alter its metabolism from growth on chitin to competing with the intestinal microbiota for the carbon sources available within its infected host.

"Our model system provides a unique opportunity to characterize how cells within a bacterial community utilize the biotic surface of chitin for biofilm formation, as a nutrient source, a platform for genetic exchange, and transmission," says Dalia. "These studies may inform novel strategies to combat clinically relevant infections and stem the spread of antibiotic resistance and virulence in diverse bacterial pathogens."

Dalia was awarded $1,894,717 earlier this year for his grant application titled “Characterizing the basic biology and mechanisms of biofilms in Vibrio cholerae” through a Maximizing Investigators' Research Award for Early Stage Investigators (R35). The MIRA, as the award is known, is presented by the National Institute of General Medical Sciences, a section of the National Institutes of Health. It provides support for an investigator's research that falls within the mission of NIGMS. The MIRA provides researchers with greater stability and flexibility which in turn enhances scientific productivity and the chances for important breakthroughs. The award is for five years.

Dalia aims to define the mechanisms of initial adherence to chitin, DNA uptake and integration during natural transformation, and metabolism during growth in chitin biofilms and in the mammalian host following transmission.

He and his lab have generated a number of tools to address these questions. Namely, they use a novel method to fluorescently label pili, which are surface appendages required for initial attachment to chitin and for DNA uptake during natural transformation. Using this new method, they can observe the dynamic nature of these pili. This is not possible by any other approach and should allow them to address their role in chitin biofilms.

This time-lapse image series shows a bacterial pilus extending and retracting.
This time-lapse image series shows a bacterial pilus extending and retracting. IU biologists developed the genetic manipulation technique that binds fluorescent dyes to the pili which allows researchers to view the pili beneath a microscope. Image by Ankur Dalia

Pili are broadly conserved, and the Dalia lab's studies will address fundamental and long-standing questions about these structures that should be applicable to many bacterial pathogens.

They have also recently improved a method for multiplex genome editing by natural transformation (MuGENT), which can be used to dissect complex biological questions where genetic redundancy poses an issue. In preliminary data, they demonstrate that this tool is well poised to dissect the metabolism of V. cholerae in chitin biofilms and in the mammalian host. The innovative approaches proposed will provide a paradigm for the study of critical and conserved processes (i.e., adherence, biofilm formation, natural transformation, and metabolism) in diverse microbial species.

  • Faculty + Staff Intranet

Department of Biology social media channels

  • Twitter
  • Facebook
  • College of Arts & Sciences
  • Department of Biology

The College of Arts & Sciences

Indiana University

Copyright © 2025 The Trustees of Indiana University

Accessibility | College Scorecard | Privacy Notice

  • About
    • Message from the Chair
    • Faculty
      • Tenured + Tenure-Track Faculty
      • Teaching Faculty
      • Research Scientists
      • Faculty Emeriti
      • Faculty Affiliates
    • Research Associates & Postdocs
    • Staff
    • Graduate Students
    • Administration
    • Offices & Centers
    • Diversity & Inclusion
      • DEI Committee
    • Outreach
      • K-12 Educators
        • Biology Summer Institute
      • K-12 Students
        • Holland Summer Science Programs
          • Jim Holland Summer Enrichment Program in Biology (SEP)
          • Jim Holland Summer Science Research Program (SSRP)
          • Jim Holland Research Initiative in STEM Education (RISE)
      • Lessons
      • Outreach Activities
      • Outreach Coordinators
    • History
      • Faculty Emeriti
      • Historical Materials
    • About Bloomington
  • Undergraduate
    • Biology
      • Biology Learning Goals
      • Biology B.S.
        • Areas of Concentration
          • Biology of Disease
          • Cell Biology and Molecular Genetics
          • Integrative and Organismal Biology
      • Biology B.A.
      • Biology Minor
    • Biotechnology
      • Biotechnology Learning Goals
        • Biotechnology BS Learning Goals
        • Biotechnology BA Learning Goals
        • Biotechnology Minor Learning Goals
      • Program Design & Resources
      • Biotechnology B.S.
      • Biotechnology B.A.
      • Biotechnology B.S./M.S.
      • Biotechnology Minor
      • Demand and Employment Analysis
    • Microbiology
      • Microbiology B.S.
      • Microbiology B.A.
      • Microbiology Minor
    • Molecular Life Sciences
    • Research & Teaching Opportunities
      • Research Programs
      • Biology X490 Independent Study
      • Undergraduate Teaching Assistant
    • Honors Program
    • Advising
    • Awards & Scholarships
    • Student Experience
    • Graduate & Professional Program Preparation
    • Career Preparation
  • Graduate
    • Evolution, Ecology, and Behavior Ph.D.
      • Steps to become EEB student
      • Curriculum
      • EEB Faculty
    • Genome, Cell, and Developmental Biology Ph.D.
      • Steps to become GCDB student
      • Curriculum
      • GCDB Faculty
    • Microbiology Ph.D.
      • Steps to become Microbiology student
      • Curriculum
      • Microbiology Faculty
    • Biotechnology M.S.
      • Program Plan + Curriculum
      • Financial Support
      • Biotechnology Faculty
    • Multidisciplinary & Affiliated Programs
    • Advising
    • Financial Support
    • Awards & Fellowships
    • Life Assistance
    • Career Preparation
    • Alumni Career Spotlights
    • How to Apply
  • Research
    • Research Faculty
    • Associated Research Faculty
    • Research Strengths
      • Behavior
      • Chromatin, chromosomes, and genome integrity
      • Developmental mechanisms and regulation in eukaryotic systems
      • Ecology
      • Eukaryotic cell biology, cytoskeleton, and signaling
      • Evolution
      • Genomics and bioinformatics
      • Microbial cell biology and environmental responses
      • Microbial interactions and pathogenesis
      • Plant molecular biology
      • Virology
    • Centers, Instrumentation, & Resources
      • Drosophila Research Resources
    • Faculty Awards
  • News & Events
    • News
    • Events Calendar
    • Seminars
    • Named Lectures
      • Distinguished Alumni Award Lecture
      • James P. Holland Lecture Series
      • Carlos O. Miller Lecture Series
      • Hermann J. Muller Award Lecture Series
      • Norman R. Pace Lecture Series
      • Tracy M. Sonneborn Lecture Series
      • Joan Wood Lecture Series
    • Discussion Groups & Journal Clubs
    • Annual GCDB Retreat
    • Annual Microbiology Retreat
      • Micro-lympics
      • Registration
    • Newsletters
      • BioNews Fall 2022
      • BioNews Archive
  • Alumni & Giving
  • Contact
  • Student Portal
    • Undergraduate
      • Biology
        • Biology B.S.
        • Biology B.A.
        • Biology Minor
      • Biotechnology
        • Biotechnology B.S.
        • Biotechnology B.A.
        • Biotechnology B.S./M.S.
        • Biotechnology Minor
      • Microbiology
        • Microbiology B.S.
        • Microbiology B.A.
        • Microbiology Minor
      • Molecular Life Sciences
      • Courses
        • Biology Exemption Exams
        • Course Scheduling
        • Course Evaluations
        • Biology X490 Independent Study
        • FAQs
      • Advising
      • Research Opportunities
      • Teaching Assistants
      • Honors Program
        • Eligibility
        • Honors Courses
        • Honors Thesis
        • Honors Faculty Advisors
        • How to Apply
      • Internships
    • Graduate
      • Evolution, Ecology, and Behavior Ph.D.
      • Genome, Cell, and Developmental Biology Ph.D.
      • Microbiology Ph.D.
      • Biotechnology M.S.
      • Multidisciplinary and Affiliated Programs
      • Courses
      • Transfer credits
      • Awards + funding
      • Financial Support
      • Teaching Support
      • Dissertation & Thesis Support
      • Services & Outreach
      • Submit News to Biology
      • Facilities & Resources
        • Computing Services
        • Constant Temperature Rooms
        • Dry Ice
        • Lactation Room
        • Room Reservations
          • All Rooms
          • Biology Bldg. 123
          • Biology Bldg. 248
          • Biology Bldg. A310
          • Biology Bldg. 422
          • Biology Bldg. 510
          • Myers Hall 115
          • Myers Hall 140
          • Myers Hall 209
          • Myers Hall 311
      • Student Academic Appointments
      • Bias incident reporting
      • Title IX incident reporting
    • Seminars
    • Ombudsperson
  • Donate

The College of Arts & Sciences